Journal: Cell reports

Article Title: Overlapping Activities of Two Neuronal Splicing Factors Switch the GABA Effect from Excitatory to Inhibitory by Regulating REST

doi: 10.1016/j.celrep.2019.03.072

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-calbindin D-28K monoclonal antibody (clone CB-955) , Acris Antibodies , Cat# AM08219SU-N; RRID: AB_1954252.

Techniques: Expressing, Plasmid Preparation, Recombinant, SYBR Green Assay, Reporter Assay, Isolation, Staining, Microarray, Clone Assay, Software

Journal: Tropical Medicine and Infectious Disease

Article Title: Immunization with Em CRT-Induced Protective Immunity against Echinococcus multilocularis Infection in BALB/c Mice

doi: 10.3390/tropicalmed7100279

Figure Lengend Snippet: Expression of Em CRT in E. multilocularis metacestode larvae. ( A ) RT-qPCR analysis of transcription level of Emcrt mRNA in PSCs and metacestode vesicle tissue. After being normalized with Emelp , the fold-change of the Emcrt mRNA transcription in PSCs was calculated relative to that in metacestode vesicles. * p < 0.05. ( B ) Analysis of Em CRT protein expression in the lysates of metacestode vesicles (Lane 1), metacestode vesicle fluid (Lane 2), PSCs (Lane 3) and the excretory–secretory products released by cultured metacestode vesicles (Lane 4) and by PSCs (Lane 5) by Western blot with anti-r Em CRT mouse sera. Equal amount of total protein (100 ug), determined by Bradford assay, was loaded for each sample.

Article Snippet: The Em CRT protein was recognized by the anti-r Em CRT mouse sera followed by a secondary anti-mouse IgG antibody conjugated with HRP (1:5000 dilution; BOSTER Biological Technology Co. LTD, Chengmai, China).

Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Western Blot, Bradford Assay

Journal: PLoS Genetics

Article Title: Ataxin-2 Regulates RGS8 Translation in a New BAC-SCA2 Transgenic Mouse Model

doi: 10.1371/journal.pgen.1005182

Figure Lengend Snippet: (A) Representative micrographs of calbindin-28k immunostaining of PCs in the cerebellum of BAC-Q22, BAC-Q72, and wild-type mice at 24 weeks of age. Note that reduction of calbindin immunoreactivity and disorganization of the PC layer are only observed in BAC-Q72 cerebella (see also ). (B) Western blot analyses show reduction of Calb1 and Pcp2 protein in BAC-Q72 mouse cerebella compared with wild-type at 24 weeks of age. Two animals per group were used for these analyses and the blots represent three independent experiments.

Article Snippet: ATXN2 mAb [(1:3000), BD Biosciences Inc.; cat# 611378], 5TF1-1C2 mAb [(1:3000), Millipore Inc.; #MAB1574], RGS8 rabbit polyclonal Ab [(1:3000), Novus Biologicals; #NBP2-20153], Calbindin-D-28K mAb [(1: 5000), Sigma Inc.; cat# C9848], PCP2 mAb [(1: 5000), Santa Cruz Inc.; cat# sc-137064], DDX6 rabbit polyclonal Abs [(1:4000), Santa Cruz Inc.; cat# sc-27127-R], PABPC1 mAb [(1:4000), Santa Cruz Inc.; cat# sc-27127-R], CUG-BP1 mAb [(1:4000), Santa Cruz Inc.; cat# sc-20003], Flag M2 mAb [(1:10,000), Sigma Inc.; cat# F3165], GFP mAb [(1:3000), Santa Cruz Inc.; cat# sc-9996] and MYC mAb conjugated with HRP [(1:5000), Invitrogen Inc.; cat# A3858].

Techniques: Immunostaining, Western Blot

Journal: Frontiers in Neural Circuits

Article Title: C1q Regulates Horizontal Cell Neurite Confinement in the Outer Retina

doi: 10.3389/fncir.2020.583391

Figure Lengend Snippet: Antibodies used in tissue analysis.

Article Snippet: Calbindin D-28k , Full-length recombinant human Calbindin D-28K , Horizontal cells; amacrine cells; retinal ganglion cells , Novus biologicals; chicken polyclonal; NBP2-50028; no RRID , 1:2000.

Techniques: Recombinant, Purification

Journal: PLoS Genetics

Article Title: Rgs6 is Required for Adult Maintenance of Dopaminergic Neurons in the Ventral Substantia Nigra

doi: 10.1371/journal.pgen.1004863

Figure Lengend Snippet: (A) Rgs6 expression revealed by immunohistofluorescence (red) together with TH (green) in mDA neurons of SNc but not VTA. Scale bar 410 µm. (B) Co-immunofluorescence analysis of TH (green) and Rgs6 or Pitx3 (red, as indicated) in SNc and VTA of adult mice. Arrowheads point to Pitx3-negative or Rgs6-negative mDA neurons in dorsal SNc. Scale bar 100 µm. (C) Triple immunofluorescence staining for TH (green), Pitx3 (red) and Calb1 (blue) on tissue sections of adult mice indicates that the majority of TH+Pitx3− cells of dSNc (arrowheads) are positive for Calb1, while TH+Pitx3+ cells of vSNc are negative for Calb1, consistent with depiction in A. Scale bar 100 µm.

Article Snippet: Primary antibodies used were against Pitx3 (rabbit home-made, 1∶400), Rgs6 (rabbit home-made 1∶25), Th (Millipore MAB318, 1∶1000), Th (Millipore AB152, 1∶500), Calb1 (R&D Systems AF3320, 1∶40), Fgf10 (Millipore ABN44, 1∶1000), Slc6a3 (Santa Cruz sc-32258, 1∶250), Drd2 (Santa Cruz sc5303, 1∶100), Aldh1a1 (Abcam ab24343, 1∶400), BDNF (Abcam ab108319, 1∶25), phospho-p27 (Abcam ab32096, 1∶50), phospho-Erk1/2 (Cell Signaling 4376, 1∶25), DJ-1 (Abcam ab18257, 1∶500) Lrrk2 (Epitomics 3514-1, 1∶50), Pink1 (Novus Biologicals BC100-494, 1∶50), LC3B (Cell Signaling 3868, 1∶50), and Vmat2 (Millipore AB1598P, 1∶200).

Techniques: Expressing, Immunohistofluorescence, Immunofluorescence, Staining